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How close to email send is audience calculation done

Question asked by b28ac3f7f5db38999377a8da940cde54862e84f3 on Sep 19, 2016
Latest reply on Sep 19, 2016 by e6615dd679b5dd0c3e60c20085706f392a9af2cb

I frequently stage emails well in advance of their send date and am wondering how close to sending is the audience calculated? Is the audience only calculated on approval? Or is it recalculated at the time of send as well?